Transgenic poplar characterized by ectopic expression of a pine cytosolic glutamine synthetase gene exhibits enhanced tolerance to water stress.
Identifieur interne : 004172 ( Main/Exploration ); précédent : 004171; suivant : 004173Transgenic poplar characterized by ectopic expression of a pine cytosolic glutamine synthetase gene exhibits enhanced tolerance to water stress.
Auteurs : Rami T. El-Khatib [États-Unis] ; Erik P. Hamerlynck ; Fernando Gallardo ; Edward G. KirbySource :
- Tree physiology [ 0829-318X ] ; 2004.
Descripteurs français
- KwdFr :
- Arbres (enzymologie), Arbres (génétique), Arbres (physiologie), Chlorophylle (physiologie), Déshydratation (MeSH), Glutamate-ammonia ligase (génétique), Photosynthèse (physiologie), Populus (enzymologie), Populus (génétique), Populus (physiologie), Régulation de l'expression des gènes codant pour des enzymes (génétique), Transpiration des plantes (physiologie), Végétaux génétiquement modifiés (enzymologie), Végétaux génétiquement modifiés (physiologie).
- MESH :
- enzymologie : Arbres, Populus, Végétaux génétiquement modifiés.
- génétique : Arbres, Glutamate-ammonia ligase, Populus, Régulation de l'expression des gènes codant pour des enzymes.
- physiologie : Arbres, Chlorophylle, Photosynthèse, Populus, Transpiration des plantes, Végétaux génétiquement modifiés.
- Déshydratation.
English descriptors
- KwdEn :
- Chlorophyll (physiology), Dehydration (MeSH), Gene Expression Regulation, Enzymologic (genetics), Glutamate-Ammonia Ligase (genetics), Photosynthesis (physiology), Plant Transpiration (physiology), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (physiology), Populus (enzymology), Populus (genetics), Populus (physiology), Trees (enzymology), Trees (genetics), Trees (physiology).
- MESH :
- chemical , genetics : Glutamate-Ammonia Ligase.
- chemical , physiology : Chlorophyll.
- enzymology : Plants, Genetically Modified, Populus, Trees.
- genetics : Gene Expression Regulation, Enzymologic, Populus, Trees.
- physiology : Photosynthesis, Plant Transpiration, Plants, Genetically Modified, Populus, Trees.
- Dehydration.
Abstract
Physiological responses to water stress in hybrid poplar (INRA 7171-B4, Populus tremula L. x P. alba L.) lines transformed to overexpress a pine cytosolic glutamine synthetase (GS1) gene were compared with those of non-transgenic plants. Before, during and after a drought treatment, net photosynthetic rates (Anet) were higher in transgenic than in non-transgenic plants. Stomatal conductance (gs) was higher in transgenic than in non-transgenic plants before, but not after exposure to drought. Before drought treatment, a sudden reduction in photosynthetic photon flux caused a greater burst of CO2 efflux in transgenic than non-transgenic plants, indicating greater photorespiratory activity. Drought caused greater reductions in photochemical quenching, photosystem II (PSII) antennae transfer efficiency (Fv'/Fm') and light-adapted PSII yield (PhiPSII) in non-transgenic than in transgenic plants, especially at low irradiances. Antennae-based thermal dissipation was higher in transgenic plants than in non-transgenic plants both during the imposition of drought and 1 or 3 days after the relief of drought. Under severe water stress and subsequently, transgenic plants maintained a higher expression of glutamine synthetase, glutamate synthase and Rubisco and higher concentrations of chlorophyll and glycine than non-transgenic plants. These findings indicate that overexpression of pine cytosolic GS1 enhanced sustained photosynthetic electron transport capacity during severe stomatal limitation. The data also suggest that ectopic expression of cytosolic GS increases photorespiratory activity, and that this serves as a protective sink for electrons from photosynthetic reaction centers.
DOI: 10.1093/treephys/24.7.729
PubMed: 15123444
Affiliations:
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El-Khatib</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, Rutgers University, Newark, NJ 07102-1811, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, Rutgers University, Newark, NJ 07102-1811</wicri:regionArea><placeName><region type="state">New Jersey</region><settlement type="city">New Brunswick (New Jersey)</settlement></placeName><orgName type="university">Université Rutgers</orgName></affiliation></author><author><name sortKey="Hamerlynck, Erik P" sort="Hamerlynck, Erik P" uniqKey="Hamerlynck E" first="Erik P" last="Hamerlynck">Erik P. Hamerlynck</name></author><author><name sortKey="Gallardo, Fernando" sort="Gallardo, Fernando" uniqKey="Gallardo F" first="Fernando" last="Gallardo">Fernando Gallardo</name></author><author><name sortKey="Kirby, Edward G" sort="Kirby, Edward G" uniqKey="Kirby E" first="Edward G" last="Kirby">Edward G. 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El-Khatib</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, Rutgers University, Newark, NJ 07102-1811, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, Rutgers University, Newark, NJ 07102-1811</wicri:regionArea><placeName><region type="state">New Jersey</region><settlement type="city">New Brunswick (New Jersey)</settlement></placeName><orgName type="university">Université Rutgers</orgName></affiliation></author><author><name sortKey="Hamerlynck, Erik P" sort="Hamerlynck, Erik P" uniqKey="Hamerlynck E" first="Erik P" last="Hamerlynck">Erik P. Hamerlynck</name></author><author><name sortKey="Gallardo, Fernando" sort="Gallardo, Fernando" uniqKey="Gallardo F" first="Fernando" last="Gallardo">Fernando Gallardo</name></author><author><name sortKey="Kirby, Edward G" sort="Kirby, Edward G" uniqKey="Kirby E" first="Edward G" last="Kirby">Edward G. 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Before, during and after a drought treatment, net photosynthetic rates (Anet) were higher in transgenic than in non-transgenic plants. Stomatal conductance (gs) was higher in transgenic than in non-transgenic plants before, but not after exposure to drought. Before drought treatment, a sudden reduction in photosynthetic photon flux caused a greater burst of CO2 efflux in transgenic than non-transgenic plants, indicating greater photorespiratory activity. Drought caused greater reductions in photochemical quenching, photosystem II (PSII) antennae transfer efficiency (Fv'/Fm') and light-adapted PSII yield (PhiPSII) in non-transgenic than in transgenic plants, especially at low irradiances. Antennae-based thermal dissipation was higher in transgenic plants than in non-transgenic plants both during the imposition of drought and 1 or 3 days after the relief of drought. Under severe water stress and subsequently, transgenic plants maintained a higher expression of glutamine synthetase, glutamate synthase and Rubisco and higher concentrations of chlorophyll and glycine than non-transgenic plants. These findings indicate that overexpression of pine cytosolic GS1 enhanced sustained photosynthetic electron transport capacity during severe stomatal limitation. The data also suggest that ectopic expression of cytosolic GS increases photorespiratory activity, and that this serves as a protective sink for electrons from photosynthetic reaction centers.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15123444</PMID><DateCompleted><Year>2006</Year><Month>02</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>7</Issue><PubDate><Year>2004</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Transgenic poplar characterized by ectopic expression of a pine cytosolic glutamine synthetase gene exhibits enhanced tolerance to water stress.</ArticleTitle><Pagination><MedlinePgn>729-36</MedlinePgn></Pagination><Abstract><AbstractText>Physiological responses to water stress in hybrid poplar (INRA 7171-B4, Populus tremula L. x P. alba L.) lines transformed to overexpress a pine cytosolic glutamine synthetase (GS1) gene were compared with those of non-transgenic plants. Before, during and after a drought treatment, net photosynthetic rates (Anet) were higher in transgenic than in non-transgenic plants. Stomatal conductance (gs) was higher in transgenic than in non-transgenic plants before, but not after exposure to drought. Before drought treatment, a sudden reduction in photosynthetic photon flux caused a greater burst of CO2 efflux in transgenic than non-transgenic plants, indicating greater photorespiratory activity. Drought caused greater reductions in photochemical quenching, photosystem II (PSII) antennae transfer efficiency (Fv'/Fm') and light-adapted PSII yield (PhiPSII) in non-transgenic than in transgenic plants, especially at low irradiances. Antennae-based thermal dissipation was higher in transgenic plants than in non-transgenic plants both during the imposition of drought and 1 or 3 days after the relief of drought. Under severe water stress and subsequently, transgenic plants maintained a higher expression of glutamine synthetase, glutamate synthase and Rubisco and higher concentrations of chlorophyll and glycine than non-transgenic plants. These findings indicate that overexpression of pine cytosolic GS1 enhanced sustained photosynthetic electron transport capacity during severe stomatal limitation. The data also suggest that ectopic expression of cytosolic GS increases photorespiratory activity, and that this serves as a protective sink for electrons from photosynthetic reaction centers.</AbstractText><CopyrightInformation>Copyright 2004 Heron Publishing</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>el-Khatib</LastName><ForeName>Rami T</ForeName><Initials>RT</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, Rutgers University, Newark, NJ 07102-1811, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hamerlynck</LastName><ForeName>Erik P</ForeName><Initials>EP</Initials></Author><Author ValidYN="Y"><LastName>Gallardo</LastName><ForeName>Fernando</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Kirby</LastName><ForeName>Edward G</ForeName><Initials>EG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>1406-65-1</RegistryNumber><NameOfSubstance UI="D002734">Chlorophyll</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.3.1.2</RegistryNumber><NameOfSubstance UI="D005974">Glutamate-Ammonia Ligase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002734" MajorTopicYN="N">Chlorophyll</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003681" MajorTopicYN="N">Dehydration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005974" MajorTopicYN="N">Glutamate-Ammonia Ligase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018526" MajorTopicYN="N">Plant Transpiration</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>5</Month><Day>5</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>2</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>5</Month><Day>5</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15123444</ArticleId><ArticleId IdType="doi">10.1093/treephys/24.7.729</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>New Jersey</li></region><settlement><li>New Brunswick (New Jersey)</li></settlement><orgName><li>Université Rutgers</li></orgName></list><tree><noCountry><name sortKey="Gallardo, Fernando" sort="Gallardo, Fernando" uniqKey="Gallardo F" first="Fernando" last="Gallardo">Fernando Gallardo</name><name sortKey="Hamerlynck, Erik P" sort="Hamerlynck, Erik P" uniqKey="Hamerlynck E" first="Erik P" last="Hamerlynck">Erik P. Hamerlynck</name><name sortKey="Kirby, Edward G" sort="Kirby, Edward G" uniqKey="Kirby E" first="Edward G" last="Kirby">Edward G. Kirby</name></noCountry><country name="États-Unis"><region name="New Jersey"><name sortKey="El Khatib, Rami T" sort="El Khatib, Rami T" uniqKey="El Khatib R" first="Rami T" last="El-Khatib">Rami T. El-Khatib</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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